LOCUS       sequence-116470-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  TITLE     Unpublished pBGSA FPR1 fluorophore tagged CDNAs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBZSA FPR1 ECFP
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(108..126)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(118..165)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(270..294)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(291..494)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(320..340)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gaagtcccgg gagacccgag ccggtcggtc cagaactcga ccactccggc gacgtcgcgc
       61 gcggtgagca ccggaacggc actggtcaac ttggccatgg tggccctcct atagtgagtc
      121 gtattatact atgccgatat actatgccga tgattaattg tcaacacgtg ctgcaggtcc
      181 gttccaatgc accgttcccg gccgcggagg ctggatcggt cccggtgtct tctatggagg
      241 tcaaaacagc gtggatggcg tctccaggcg atctgacggt tcactaaacg agctctgctt
      301 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
      361 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
      421 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
      481 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
      541 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
      601 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
      661 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
      721 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
      781 accgtaag
//
