LOCUS       sequence-116480-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of SUMO2 with a strep and an HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Bursomanno S, McGouran JF, Kessler BM, Hickson ID, Liu Y
  TITLE     Regulation of SUMO2 target proteins by the proteasome in human cells
            exposed to replication stress.
  JOURNAL   J Proteome Res. 2015 Apr 3;14(4):1687-99. doi: 10.1021/pr500997p. 
            Epub 2015 Mar 19.
  PUBMED    25748227
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr500997p";
            journalName: "J Proteome Res"; date: "2015-04-3- 3"; volume: "14"; 
            issue: "4"; pages: "1687-99"
COMMENT     Sequence Label: pcDNA3.1-StrepHA-SUMO2
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..305)
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 2"
                     /label=SUMO2
                     /translation="MADEKPKEGVKTENNNHINLKVAGQDGSVVQFKIKRHTPLSKLMK
                     AYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG"
     CDS             complement(321..347)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(375..398)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(435..458)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(508..527)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(509..527)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(572..671)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(601..621)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tagactcgag cggccgctca gtagacacct cccgtctgct gttggaacac atcaattgta
       61 tcttcatcct ccatttccaa ctgtgcaggt gtgtctgttt cattgattgg ttgcccgtca
      121 aatcggaatc tgatctgcct cattgacaat ccctgtcgtt cacaataggc tttcattagt
      181 ttactaagtg gtgtatgcct cttaatctta aactgcacca cagaaccatc ctgccccgcc
      241 accttcaaat taatatgatt gttgttctca gtcttgactc cttccttggg cttttcgtcg
      301 gccatgatat ccgctgcagc ggcgtaatcg gggacgtcat aagggtaaga attcgggacc
      361 gcggtctcgg cgcctttttc gaactgcggg tggctccacg atccacctcc cgatccacct
      421 ccggaacctc cacctttctc gaactgcggg tggctccagc tagccatggt accaagctta
      481 agtttaaacg ctagccagct tgggtctccc tatagtgagt cgtattaatt tcgataagcc
      541 agtaagcagt gggttctcta gttagccaga gagctctgct tatatagacc tcccaccgta
      601 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
      661 gttgattttg gtgccaaaac aaactcccat tgacgtcaat
//
