LOCUS       sequence-116481-         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET biosensor Destination vector for yeast expression. Encodes a 
            FRET pair for fusion with ligand binding domain of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Jones AM, Danielson JA, Manojkumar SN, Lanquar V, Grossmann G, 
            Frommer WB
  TITLE     Abscisic acid dynamics in roots detected with genetically encoded 
            FRET sensors.
  JOURNAL   eLife. 2014 Apr 15;3:e01741. doi: 10.7554/eLife.01741.
  PUBMED    24737862
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-04-15"; volume: "3:e01741. doi"; pages: " 
            10.7554/eLife.01741"
COMMENT     Sequence Label: pDRFLIP32
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             55..77
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             97..114
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             118..150
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(154..162,163..177)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 cacacattca aaagaaagaa aaaaaatata ccccagcctc gatctagaaa taattttgtt
       61 taactttaag aaggagatat acatatgcgg ggttctcatc atcatcatca tcatggtatg
      121 gctagcatga ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggat
      181 ccgggcctcg aggtttctaa aggtgaagaa ttgtttacgg gcgtcgtccc gatcctcgtg
      241 gaactcgacg gggatgttaa cgggcataag ttttcggtca gcggggaagg ggagggggac
      301 gcgacgtatg ggaagctcac tctcaagctg atctgtacga cggggaaact cccggtcccg
      361 tggccgacgc tggtcacgac gctgggatac gggctccaat gctttgcgag gtatccggac
      421 cacatgaaac agcatgactt tttcaaatcg gcgatgccgg agggatacgt gcaggaacgg
      481 acgatctttt tcaaagacga tgggaactat aagacgcggg cggaagtcaa gtttgaaggg
      541 gacacgctcg tcaaccggat cgaactcaag gggattgact tcaaagagga tgggaacata
      601 ctcggccata agctcgaata caattacaac tcgcataacg tatacatcac cgcggataag
      661 caaaagaacg ggatcaaagc caatttcaaa
//
