LOCUS       sequence-116486-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET biosensor Destination vector for E. coli expression. Encodes a 
            FRET pair for fusion with ligand binding domain of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Jones AM, Danielson JA, Manojkumar SN, Lanquar V, Grossmann G, 
            Frommer WB
  TITLE     Abscisic acid dynamics in roots detected with genetically encoded 
            FRET sensors.
  JOURNAL   eLife. 2014 Apr 15;3:e01741. doi: 10.7554/eLife.01741.
  PUBMED    24737862
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-04-15"; volume: "3:e01741. doi"; pages: " 
            10.7554/eLife.01741"
COMMENT     Sequence Label: pFLIP36
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..46
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             50..82
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(86..94,95..109)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    824..848
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agaaggagat atacatatgc ggggttctca tcatcatcat catcatggta tggctagcat
       61 gactggtgga cagcaaatgg gtcgggatct gtacgacgat gacgataagg atccgggcct
      121 cgaggtttct aaaggtgaag aattgtttac gggcgtcgtc ccgatcctcg tggaactcga
      181 cggggatgtt aacgggcata agttttcggt cagcggggaa ggggaggggg acgcgacgta
      241 tgggaagctc actctcaagc tgatctgtac gacggggaaa ctcccggtcc cgtggccgac
      301 gctggtcacg acgctgggat acgggctcca atgctttgcg aggtatccgg accacatgaa
      361 acagcatgac tttttcaaat cggcgatgcc ggagggatac gtgcaggaac ggacgatctt
      421 tttcaaagac gatgggaact ataagacgcg ggcggaagtc aagtttgaag gggacacgct
      481 cgtcaaccgg atcgaactca aggggattga cttcaaagag gatgggaaca tactcggcca
      541 taagctcgaa tacaattaca actcgcataa cgtatacatc accgcggata agcaaaagaa
      601 cgggatcaaa gccaatttca aaatccggca taacatagag gatggggggg tccaactggc
      661 ggatcactat cagcaaaaca cgccgatagg ggatgggccg gtcctcctcc cggataacca
      721 ttacctctcg taccaaagcg cgctctcgaa ggacccgaat gagaaacggg accacatggt
      781 tctcctggag ttcgtcacgg cggcgggcat aggtaccgat atcacaagtt tgtacaaaaa
      841 agctgaacga gaaacgtaaa atgat
//
