LOCUS       sequence-116487-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET biosensor Destination vector for E. coli expression. Encodes a 
            FRET pair for fusion with ligand binding domain of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Jones AM, Danielson JA, Manojkumar SN, Lanquar V, Grossmann G, 
            Frommer WB
  TITLE     Abscisic acid dynamics in roots detected with genetically encoded 
            FRET sensors.
  JOURNAL   eLife. 2014 Apr 15;3:e01741. doi: 10.7554/eLife.01741.
  PUBMED    24737862
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-04-15"; volume: "3:e01741. doi"; pages: " 
            10.7554/eLife.01741"
COMMENT     Sequence Label: pFLIP35
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..60)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    752..776
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggctttgtta gcagccggat cgggccctta taaatcttcc tcacttatca atttttgttc
       61 ggagttgcgg gccacggcgc tctcgtaaac ggtcaccttg ttgtagtcct tgtcgtggtt
      121 caggatctcg atgcggtggt ccacaaagtg atagtcgggc agcttcaccg ccttcttggc
      181 cctgtagatg gtcttgaagt caacgcggtg gtggccgccg ccctccagca gcagcttgtg
      241 cttgacgtcg cccttcagca cgccgtcgcg cacgtacatc ctctcggtgg aggcgtccca
      301 gccggtggtc ttcttctgca tcacggggcc gttggggggg aagttctcgc ccttgaggtg
      361 tatctcgtag atgaaggagt cctcctccat ggagatgtcg gacttcacct tcacgatgcc
      421 cttgtcctcg aaggtcatgg tgcgctccca agagtagccc tcggggaagg actgcttgaa
      481 gtagttgggg atgtcgtcgg ggtacttggt gaaggccctg ttgccgtagg cgaacgcggt
      541 ggtcagaatg tcgtaggaga agggcagggg ggctccctcc ttcacctcca ggttgatggt
      601 gttggtgccg tcgtagggct tgccctcgcc ctcgccctcg atcacgaagg cgtggccatt
      661 cacgttgccc tccatcttca gcttgatctt catgtcgggc ttgattacgc ccattgtggt
      721 ctcctcgccc ttgctcacac tagtcgatat caccactttg tacaagaaag ctgaacgaga
      781 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
      841 ataatactgt aa
//
