LOCUS       sequence-116498-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Basic Part: CDS - Controller protein, lacI repressor (in 
            concert with CAP, represses pLacI, R0010). Modified to fix illegal 
            site. [C:C0012m:D].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: C0012m (lacI)_CD
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     86..103
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    109..129
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(147..179)
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
ORIGIN
        1 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
       61 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcct gcagcggccg
      121 ctactagtag gtctctacct ttattaagct actaaagcgt agttttcgtc gtttgcagcc
      181 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
      241 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg
      301 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
      361 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
      421 gagctatctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
      481 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
      541 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
      601 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
      661 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
      721 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcctc atgggagaaa
      781 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
      841 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagtta
//
