LOCUS       sequence-116500-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Basic Part: CDS - Controller protein, araC repressor/activator
            (activates pBAD, I13453) [C:C0080:D].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: C0080 (araC)_CD
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     85..102
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    108..128
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(146..178)
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
ORIGIN
        1 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
       61 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcctg cagcggccgc
      121 tactagtagg tctctacctt tattaagcta ctaaagcgta gttttcgtcg tttgcagctg
      181 acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc
      241 tggccccggt gcatttttta aatactcgcg agaaatagag ttgatcgtca aaaccaacat
      301 tgcgaccgac ggtggcgata ggcatccggg tagtgctcaa aagcagcttc gcctggctga
      361 tgcgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg
      421 acagacgcga cggcgacagg caaacatgct gtgcgacgct ggcgatatca aaattgctgt
      481 ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat
      541 ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg
      601 ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt
      661 cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa acccgtattg gcaaatattg
      721 acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat
      781 accattcgcg agcctccgga tgacgaccgt agtgatgaa
//
