LOCUS       sequence-116505-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET biosensor Destination vector for E. coli expression. Encodes a 
            FRET pair for fusion with ligand binding domain of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Jones AM, Danielson JA, Manojkumar SN, Lanquar V, Grossmann G, 
            Frommer WB
  TITLE     Abscisic acid dynamics in roots detected with genetically encoded 
            FRET sensors.
  JOURNAL   eLife. 2014 Apr 15;3:e01741. doi: 10.7554/eLife.01741.
  PUBMED    24737862
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-04-15"; volume: "3:e01741. doi"; pages: " 
            10.7554/eLife.01741"
COMMENT     Sequence Label: pFLIP30
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..36
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..72
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(76..84,85..99)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 atacatatgc ggggttctca tcatcatcat catcatggta tggctagcat gactggtgga
       61 cagcaaatgg gtcgggatct gtacgacgat gacgataagg atccgggcct cgaggtttct
      121 aaaggtgaag aattgtttac gggcgtcgtc ccgatcctcg tggaactcga cggggatgtt
      181 aacgggcata agttttcggt cagcggggaa ggggaggggg acgcgacgta tgggaagctc
      241 actctcaagc tgatctgtac gacggggaaa ctcccggtcc cgtggccgac gctggtcacg
      301 acgctgggat acgggctcca atgctttgcg aggtatccgg accacatgaa acagcatgac
      361 tttttcaaat cggcgatgcc ggagggatac gtgcaggaac ggacgatctt tttcaaagac
      421 gatgggaact ataagacgcg ggcggaagtc aagtttgaag gggacacgct cgtcaaccgg
      481 atcgaactca aggggattga cttcaaagag gatgggaaca tactcggcca taagctcgaa
      541 tacaattaca actcgcataa cgtatacatc accgcggata agcaaaagaa cgggatcaaa
      601 gccaatttca aaatccggca taacatagag gatggggggg tccaactggc ggatcactat
      661 cagcaaaaca cgccgatagg ggatgggccg gtcctcctcc cggataacca ttacctctcg
      721 taccaaagcg cgctctcgaa ggacccgaat
//
