LOCUS       sequence-116517-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Parent vector for constructing non-replicating lesion-bearing 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   You C, Wang Y
  TITLE     Quantitative measurement of transcriptional inhibition and 
            mutagenesis induced by site-specifically incorporated DNA lesions in
            vitro and in vivo.
  JOURNAL   Nat Protoc. 2015 Sep;10(9):1389-406. doi: 10.1038/nprot.2015.094. 
            Epub 2015 Aug 20.
  PUBMED    26292071
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.094"; journalName: "Nat Protoc"; date: 
            "2015-09"; volume: "10"; issue: "9"; pages: "1389-406"
COMMENT     Sequence Label: pTGFP-T7-Hha10T
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      146..155
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             152..850
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRINGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     primer_bind     complement(1010..1029)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtctaata cgactcacta tagggagatc cgctagcgga
       61 tgcatcgact caattatagc acgccatgga tgactcgctg cgaattctgc agtcgacggt
      121 accgcgggcc cgggatccac cggtcgccac catggagagc gacgagagcg gcctgcccgc
      181 catggagatc gagtgccgca tcaacggcac cctgaacggc gtggagttcg agctggtggg
      241 cggcggagag ggcacccccg agcagggccg catgaccaac aagatgaaga gcaccaaagg
      301 cgccctgacc ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt
      361 cggcacctac cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta
      421 caccaacacc cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta
      481 ccgctacgag gccggccgcg tgatcggcga cttcaaggtg atgggcaccg gcttccccga
      541 ggacagcgtg atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca
      601 ccccatgggc gataacgatc tggatggcag cttcacccgc accttcagcc tgcgcgacgg
      661 cggctactac agctccgtgg tggacagcca catgcacttc aagagcgcca tccaccccag
      721 catcctgcag aacgggggcc ccatgttcgc cttccgccgc gtggaggagg atcacagcaa
      781 caccgagctg ggcatcgtgg agtaccagca cgccttcaag accccggatg cagatgccgg
      841 tgaagaataa agcggccgca actctagatc ataatcagcc ataccacatt tgtagaggtt
      901 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
      961 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     1021 acaaatttca caaataaagc atttttt
//
