LOCUS       sequence-116518-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Competitor vector for transcriptional mutagenesis assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   You C, Wang Y
  TITLE     Quantitative measurement of transcriptional inhibition and 
            mutagenesis induced by site-specifically incorporated DNA lesions in
            vitro and in vivo.
  JOURNAL   Nat Protoc. 2015 Sep;10(9):1389-406. doi: 10.1038/nprot.2015.094. 
            Epub 2015 Aug 20.
  PUBMED    26292071
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.094"; journalName: "Nat Protoc"; date: 
            "2015-09"; volume: "10"; issue: "9"; pages: "1389-406"
COMMENT     Sequence Label: pTGFP-T7-Hha10comp
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     33..52
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        33..51
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      156..165
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             162..860
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRINGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     primer_bind     complement(1020..1039)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tctaatacga ctcactatag ggagatccgc
       61 tagcggatgc atcgactcaa ttatagcata tcgccatgga tgactcgctg cgaattctgc
      121 agtcgacggt accgcgggcc cgggatccac cggtcgccac catggagagc gacgagagcg
      181 gcctgcccgc catggagatc gagtgccgca tcaacggcac cctgaacggc gtggagttcg
      241 agctggtggg cggcggagag ggcacccccg agcagggccg catgaccaac aagatgaaga
      301 gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg ggctacggct
      361 tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac gccatcaaca
      421 acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga
      481 gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg atgggcaccg
      541 gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac gccaccgtgg
      601 agcacctgca ccccatgggc gataacgatc tggatggcag cttcacccgc accttcagcc
      661 tgcgcgacgg cggctactac agctccgtgg tggacagcca catgcacttc aagagcgcca
      721 tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc gtggaggagg
      781 atcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag accccggatg
      841 cagatgccgg tgaagaataa agcggccgca actctagatc ataatcagcc ataccacatt
      901 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      961 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     1021 caatagcatc acaaatttca caaataaagc atttttt
//
