LOCUS       sequence-116522-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ITPKA-CNL in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pCNL-N1_ITPKA
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      226..235
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(232..234,235..237,238..750)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(277..298)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(538..557)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     885..906
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgagctca agcttatggg catggcgcga ccacggggcg cggggccctg cagccccggg
      121 ttggagcggg ctccgcgccg gagcgtcggg gagctgcgcc tgctcttcga agcgcgctgc
      181 gccgcagtcg ccgccgcagc ggccgcaggg tcggatccac cggtcgccac catggtgagc
      241 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      301 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      361 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      421 accctgtcct ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
      481 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
      541 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      601 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
      661 tacaactact ttagcgacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag
      721 gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac
      781 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
      841 acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
      901 ttcgtgaccg ccgccggggg taccaaggtg tacgaccccg agcagaggaa gaggatgatc
      961 accggcccc
//
