LOCUS       sequence-116534-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Vinculin-CNL in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pCNL-N1_vinculin
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     118..139
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      181..190
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct
       61 tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt
      121 cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca
      181 ccatggtggc gaccggtgga tcccgggccc gcggtaccgt cgacggctgg taccagggag
      241 tctttctgac ccagcgcaga gtaaagccag catctgttcg tattttgatt gaagtagctt
      301 cagcctctcg cacagtctcc ttcacagact gcatgaggtt ctgggcatta tgaaccagca
      361 tctctgtggc ctgctcagac tcctcatcac tgatgttggt ccggcccagc atagtggcct
      421 tcactgtgga taggattttg agctgggtgc ttatagttgg gattcgctcg catacctgta
      481 agagattggt tctaatccgc ttatctgtgc actgcttggc aacctccttg gccaacctcg
      541 tcacctcatc agaggccttg gcgatatcct tggcacactg aataagtgcc cgcttggtac
      601 cactgccccc tcttaccagc cgagacatct ctgccatcag cagagccatg cgcttggctg
      661 ctgcaatgat gtcattgccc ttgctagacc atttccgagc ttcatcgtgg agctgcctgg
      721 cggccatcat cattggctgg ttaatcacct caccagcttt ctgctcaggg aactcttcat
      781 ccttctcttc tggtggtggg ggcctgggtg gagggacttc accctcaggc agaggtggct
      841 taggaggagc cagctcatca gttagtcgta gctgttcaag gtctggtgga ggaggcggga
      901 agtcaggctc ctgaggttgg aaggcttctc tgaccttggc cacagctccc aggatccgat
      961 atcctgagtc caggaagctc ttttgcaggc cagggtcaga
//
