LOCUS       sequence-116542-         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ITPKA-ONL in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pONL-N1_ITPKA
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      226..235
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             232..885
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             232..885
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgagctca agcttatggg catggcgcga ccacggggcg cggggccctg cagccccggg
      121 ttggagcggg ctccgcgccg gagcgtcggg gagctgcgcc tgctcttcga agcgcgctgc
      181 gccgcagtcg ccgccgcagc ggccgcaggg tcggatccac cggtcgccac catggtgagc
      241 gtgatcaagc ccgagatgaa gatgaggtac tacatggacg gctccgtcaa tgggcatgag
      301 ttcacaatcg agggtgaggg cacaggcaga ccttacgagg gacatcagga gatgacactg
      361 cgcgtcacaa tggccgaggg cgggccaatg cctttcgcct tcgacctggt gtcccacgtg
      421 ttctgttacg gccacagagt ttttactaaa tatccagaag agatcccaga ctatttcaag
      481 caggcctttc ctgagggcct gtcctgggag aggtccctgg agttcgagga cggcggctcc
      541 gcctccgtga gcgcccacat cagcctgagg ggcaacacct tctaccacaa gtccaagttc
      601 accggcgtga acttccccgc cgacggcccc atcatgcaga accagagcgt ggactgggag
      661 ccctccaccg agaagatcac cgccagcgac ggcgtgctga agggcgacgt gaccatgtac
      721 ctgaagctgg agggcggcgg caaccacaag tgccagatga agaccaccta caaggccgcc
      781 aaggagatcc tggagatgcc cggcgaccac tacatcggcc acaggctggt gaggaagacc
      841 gagggcaaca tcaccgagca ggtggaggac gccgtggccc actccggtac caaggtgtac
      901 gaccccgagc aacgcaaacg catgatcact gggcctcagt ggtgggctcg ctgcaagcaa
      961 atgaacgtgc tggactcctt catca
//
