LOCUS       sequence-116547-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET biosensor Destination vector for yeast expression. Encodes a 
            FRET pair for fusion with ligand binding domain of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Jones AM, Danielson JA, Manojkumar SN, Lanquar V, Grossmann G, 
            Frommer WB
  TITLE     Abscisic acid dynamics in roots detected with genetically encoded 
            FRET sensors.
  JOURNAL   eLife. 2014 Apr 15;3:e01741. doi: 10.7554/eLife.01741.
  PUBMED    24737862
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-04-15"; volume: "3:e01741. doi"; pages: " 
            10.7554/eLife.01741"
COMMENT     Sequence Label: pDRFLIP36
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(45..74)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(join(75..785,786..788))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(117..138)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     466..485
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     725..746
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    802..826
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtatcgata agcttgatat cgaattcctg cagcccggcc cttataaatc ttcctcactt
       61 atcaattttt gttccttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
      121 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg
      181 gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
      241 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttg
      301 gccttgatgc cgttcttctg cttgtcggcg gtgatataga cgttgtcgct gatggcgttg
      361 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
      421 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
      481 tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag
      541 aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc actgcacgcc ccaggtcagg
      601 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg
      661 gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg
      721 tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc
      781 ttgctcacac tagtcgatat caccactttg tacaagaaag ctgaacgaga aacgtaaaat
      841 gatataaata
//
