LOCUS       sequence-116551-         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for S. aureus Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, 
            Gonzales AP, Li Z, Peterson RT, Yeh JJ, Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
  JOURNAL   Nature. 2015 Jun 22. doi: 10.1038/nature14592.
  PUBMED    26098369
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Jun 22. doi: 10.1038/nature14592."
COMMENT     Sequence Label: BPK2101
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     92..111
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        92..110
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    111..135
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc
       61 tctcccttat gcgactcctg cattaggaaa ttaatacgac tcactatagg ggaattgtga
      121 gcggataaca attcccctgt agaaataatt ttgtttaact ttaataagga gatataccat
      181 gggcaaacgg aactacatcc tggggcttga cattgggata accagcgttg gctacggaat
      241 tattgattat gagacacgcg atgtgattga cgccggggtt aggctgttca aagaggccaa
      301 cgttgaaaac aacgagggaa gacggagtaa gcgcggagca agaagactca agcgcagacg
      361 gagacatcgg attcagaggg tgaaaaagct gctcttcgat tacaatctcc tgaccgatca
      421 tagtgagctg agcggaatca acccctacga ggcgcgagtg aaagggcttt cccagaagct
      481 gtccgaagag gagttctccg ccgcgttgct gcacctggcc aaacggaggg gggttcacaa
      541 tgtaaacgaa gtggaggagg acacgggcaa tgaacttagt acgaaagaac agatcagtag
      601 gaactctaag gctctcgaag agaaatacgt cgctgagttg cagcttgaga gactgaaaaa
      661 agacggcgaa gtacgcggat ctattaatag gttcaagact tcagattacg taaaggaagc
      721 caagcagctc ctgtaagtac agaaagcgta ccatc
//
