LOCUS       sequence-116553-         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for S. aureus Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, 
            Gonzales AP, Li Z, Peterson RT, Yeh JJ, Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
  JOURNAL   Nature. 2015 Jun 22. doi: 10.1038/nature14592.
  PUBMED    26098369
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Jun 22. doi: 10.1038/nature14592."
COMMENT     Sequence Label: BPK2101
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(239..286)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     282..300
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     primer_bind     complement(476..495)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(477..495)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(565..630)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(637..657)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac gcctggttgc
       61 tacgcctgaa taagtgataa taagcggatg aatggcagaa attcgaaagc aaattcgacc
      121 cggtcgtcgg ttcagggcag ggtcgttaaa tagccgctta tgtctattgc tggtttaccg
      181 gtttattgac taccggaagc agtgtgaccg tgtgcttctc aaatgcctga ggtttcagca
      241 aaaaacccct caagacccgt ttagaggccc caaggggtta tgctagttat tgctcagcgg
      301 atctcgccaa caagttgacg agataaacac ggcattttgc cttgttttag tagattctgt
      361 aattttaggt ataagtacaa ttttcgtcta cctcatacct aaaattacag agtactaaaa
      421 cggagaccac taggtgttgg tgaatctgtt ccgaacaacg ctatggcatg ggtctcccta
      481 tagtgagtcg tattaatttc gattatgcgg ccgtgtacaa tacgattact ttctgttcga
      541 cttaagcatt atgcggccgc tttacttgtc atcgtcatcc ttgtaatcga tgtcatgatc
      601 tttataatca ccgtcatggt ctttgtagtc gctcgagact ttcctcttct tcttggggga
      661 tccacctttt ttaatga
//
