LOCUS       sequence-116555-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression vector for SpCas9 EQR variant: 
            CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, 
            Gonzales AP, Li Z, Peterson RT, Yeh JJ, Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
  JOURNAL   Nature. 2015 Jun 22. doi: 10.1038/nature14592.
  PUBMED    26098369
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Jun 22. doi: 10.1038/nature14592."
COMMENT     Sequence Label: MSP680
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..49)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(99..119)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggtgagagac cggtcatcct gcagccttgt catcgtcatc cttgtaatcg atgtccatga
       61 tctttataat caccgtcatg gtctttgtag tcgctcgaga ctttcctctt cttcttgggg
      121 gatccgtcac ccccaagctg tgacaaatct atccgagttt catataatcc cgtgatggat
      181 tggtgaatca gtgtcgcgtc tagcacctcc ttggtagatc tgtactgttt gcgatctatc
      241 gttgtgtcaa aatacttgaa tgcggctgga gcgccgaggt tggtaagagt aaacaaatgg
      301 ataatatttt ccgcctgctc acgtatgggt ttatccctgt gcttgttgta tgcgcttaat
      361 actttgtcca gattggcatc agctaggatg actctcttac tgaattccga aatttgctct
      421 atgatttcgt cgagataatg tttgtgctgc tcaacaaaaa gttgcttctg ttcgttatct
      481 tcaggtgaac ctttcaacnt ctcgtaatgg gacgctaaat acaggaaatt cacgtattta
      541 gacggtagtg cgagttcgtt ccccttttga agctctccgg cgctagccaa catccgtttt
      601 cggccatttt ctaactcaaa cagactatac tttggtagtt taattatgag atcctttttt
      661 acttccttgt aacctttcgc ctcaaggaag tcgatggggt tcttttcaaa agacgagcgc
      721 tccataatcg ttatccccaa taattctttg actgacttca gtttcttgga ttttcccttc
      781 tcaacttttg ccactactan gacagaatag gcaactgtag ggctctcgaa gccaccgtac
      841 tttttcgggt cccagtcctt tttacga
//
