LOCUS       sequence-116556-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible lentiviral expression, TRE-gateway-HA; PGK-rtTA-2A-puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  TITLE     Root Lab pLEX and pLIX Gateway backbones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLIX_402
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(34..42,43..78,79..81,82..117,118..120,
                     121..156,157..162,163..780))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 agaatacgat atcttggatc gatccttact tagttacccg gggagcatgt caaggtcaaa
       61 atcgtcaaga gcgtcagcag gcagcatatc aaggtcaaag tcgtcaaggg catcggctgg
      121 gagcatgtct aagtcaaaat cgtcaagggc gtcggtcggc ccgccgcttt cgcactttag
      181 ctgtttctcc aggccacata tgattagttc caggccgaaa aggaaggcag gttcggctcc
      241 ctgccggtcg aacagctcaa ttgcttgtct cagaagtggg ggcatagaat cggtggtagg
      301 tgtctctctt tcctcttttg ctacttgatg ctcctgttcc tccaatacgc agcccagtgt
      361 aaagtggccc acggcggaca gagcgtacag tgcgttctcc agggagaagc cttgctgaca
      421 caggaacgcg agctgatttt ccagggtttc gtactgtttc tctgttgggc gggtgccgag
      481 atgcacttta gccccgtcgc gatgtgagag gagagcacag cggtatgact tggcgttgtt
      541 ccgcagaaag tcttgccatg actcgccttc cagggggcag aagtgggtat gatgcctgtc
      601 cagcatctcg attggcaggg catcgagcag ggcccgcttg ttcttcacgt gccagtacag
      661 ggtaggctgc tcaactccca gcttttgagc gagtttcctt gtcgtcaggc cttcgatacc
      721 gacaccattg agtaattcca gagctccgtt tatgactttg ctcttgtcca gtctagacat
      781 tggaccagg
//
