LOCUS       sequence-116559-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synchronize trafficking of TNF from the ER (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: Str-KDEL_TNF-SBP-EGFP
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..126)
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 gctcacacct gcaggtggtt cacgttgacc ttgtgggtga tgctccagtc gtgcacgaag
       61 ttgttcaagt tccccagcca gtccttcaac aacgtgtcca cctcgccaac cagtggtctt
      121 ctcgtcggaa ttcaaaggcc taggtgcgcc atgagctcgg ccctcgaggc ccagagcaat
      181 gatgccaaag tagacctggc cagattcggc aaagtcgagg tagtcgggtc gattgatctc
      241 agcactcagt ctatcgccct tctccagctg gaacacgcct ccgaggtaga tgggttcgta
      301 ccaaggttta gcctcagcgc cctcaggtgt ttcccgctga catggggatt tgatggcaga
      361 gagcagattg actttagtct ggtaagacac ggcgatccgt gaaattgtgt gtgtgagcag
      421 cacatgtgta gaaggacatc cctggccctt aaacagcacc tgggagtaga tgaggtacag
      481 tccctcacta ggcacgacga gctggttatc ccggagttcc acgccattgg cgagcagagc
      541 gtttgccctc ctattgagcc actggagctg gccctcggcc tgtgggttgg cgacgacatg
      601 ggcgacaggt ttgtcggatg gagtccgaga tgaagatcgg acagcctgtg ccagtggtga
      661 aatgagtgac agatcccggg gaaattcctc tcgctgtggt ccaatcacgc cgaaatggag
      721 cagacagaag agtgttgtag cgccagccac gatgagaaaa gagaacagag acag
//
