LOCUS       sequence-116560-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation, Inducible lentiviral expression, TRE-gateway; 
            PGK-rtTA-2A-puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  TITLE     Root Lab pLEX and pLIX Gateway backbones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCW57.1
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     202..219
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    336..357
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(372..377,378..395,396..402)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    410..426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     415..437
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     434..450
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     434..450
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     469..489
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        471..489
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        517..743
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
       61 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      121 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      181 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
      241 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
      301 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
      361 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
      421 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
      481 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
      541 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
      601 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
      661 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
      721 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgg
//
