LOCUS       sequence-116569-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation, Inducible lentiviral expression, TRE-gateway; 
            PGK-rtTA-2A-puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  TITLE     Root Lab pLEX and pLIX Gateway backbones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCW57.1
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(30..38,39..74,75..77,78..113,114..116,
                     117..152,153..158,159..776))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(777..830)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tacgatatct tggatcgatc cttacttagt tacccgggga gcatgtcaag gtcaaaatcg
       61 tcaagagcgt cagcaggcag catatcaagg tcaaagtcgt caagggcatc ggctgggagc
      121 atgtctaagt caaaatcgtc aagggcgtcg gtcggcccgc cgctttcgca ctttagctgt
      181 ttctccaggc cacatatgat tagttccagg ccgaaaagga aggcaggttc ggctccctgc
      241 cggtcgaaca gctcaattgc ttgtctcaga agtgggggca tagaatcggt ggtaggtgtc
      301 tctctttcct cttttgctac ttgatgctcc tgttcctcca atacgcagcc cagtgtaaag
      361 tggcccacgg cggacagagc gtacagtgcg ttctccaggg agaagccttg ctgacacagg
      421 aacgcgagct gattttccag ggtttcgtac tgtttctctg ttgggcgggt gccgagatgc
      481 actttagccc cgtcgcgatg tgagaggaga gcacagcggt atgacttggc gttgttccgc
      541 agaaagtctt gccatgactc gccttccagg gggcagaagt gggtatgatg cctgtccagc
      601 atctcgattg gcagggcatc gagcagggcc cgcttgttct tcacgtgcca gtacagggta
      661 ggctgctcaa ctcccagctt ttgagcgagt ttccttgtcg tcaggccttc gataccgaca
      721 ccattgagta attccagagc tccgtttatg actttgctct tgtccagtct agacattgga
      781 ccagggtttt cttcaacatc accacaagtg aggagagaac ctctaccttc ggcaccg
//
