LOCUS       sequence-116574-         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter of mouse Nanog 3'UTR with the miR-34 target site
            mutated.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Choi YJ, Lin CP, Ho JJ, He X, Okada N, Bu P, Zhong Y, Kim SY, 
            Bennett MJ, Chen C, Ozturk A, Hicks GG, Hannon GJ, He L
  TITLE     miR-34 miRNAs provide a barrier for somatic cell reprogramming.
  JOURNAL   Nat Cell Biol. 2011 Oct 23;13(11):1353-60. doi: 10.1038/ncb2366.
  PUBMED    22020437
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2011-10-23- 23"; volume: "13"; issue: "11"; 
            pages: "1353-60"
COMMENT     Sequence Label: Luc-Nanog-3'UTRmut
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..49
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggccggccgc cccgactcta gatcaccact ttgtacaaga aagctgggtc ggcgcgccca
       61 ccctttcagg aggcaaagat aagtgggcag atctctgagt tcaaggccag gctggtctgc
      121 agagctagtt caaagacaat tagagctatg cagagaaacc ctgtcttgat aaacaaagag
      181 caaataataa ttaaaaaaat aaaccagggg ctggtgagat ggctcagtgg ataagagcac
      241 ccgactgctc ttccgaaggt caggagttca aatcccagca accacatggt ggctcacaac
      301 catacgtaac aagatctgac gccctcttcg aagacagcta cagtgtactt acatataatg
      361 aataaataaa tctttaaaaa aaatatgaaa atatttggaa gaaggaagga acctggcttt
      421 gccctgactt taagcccaga tgttgcgtaa gtctcatatt tcacctggtg gagtcacaga
      481 gtagttcagg aataattcca aggcttgtgg ggtgctaaaa tgcgcatggc tttccctagt
      541 ggcttccaaa ttcacctcca aatcactggc agagaagttt tgctgcaact gtacgtaagg
      601 ctgcagaaag tcctccccga agttatggag cggagcagca ttccaaggct ggccgttcca
      661 ggactgagcg gtccaggcct ggctgctcca agttgggttg gtccaggtct ggttgttcca
      721 agttgggttg gtccaggtct ggctgctcca agttgggttg gtccaagtct gg
//
