LOCUS       sequence-116581-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Parent vector for constructing non-replicating lesion-bearing 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   You C, Wang Y
  TITLE     Quantitative measurement of transcriptional inhibition and 
            mutagenesis induced by site-specifically incorporated DNA lesions in
            vitro and in vivo.
  JOURNAL   Nat Protoc. 2015 Sep;10(9):1389-406. doi: 10.1038/nprot.2015.094. 
            Epub 2015 Aug 20.
  PUBMED    26292071
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.094"; journalName: "Nat Protoc"; date: 
            "2015-09"; volume: "10"; issue: "9"; pages: "1389-406"
COMMENT     Sequence Label: pTGFP-T7-Hha10T
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     143..163
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(188..292)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      319..774
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(406..425)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    781..902
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(792..811)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     846..865
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc
       61 tgtctcttga tcgatctttg caaaagccta ggatccatgc tttgcatact tctgcctgct
      121 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg gttctttccg
      181 cctcaggact tttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
      241 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
      301 cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
      361 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
      421 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
      481 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
      541 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
      601 tcacgttctt taatagtgga cttttgttcc aaactggaac aacactcaac cctatctcgg
      661 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
      721 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttacgcct
      781 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      841 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      901 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
      961 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcta gagttgcggc
     1021 cgctttattc ttcaccggca tctg
//
