LOCUS       sequence-116584-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Godar S, Ince TA, Bell GW, Feldser D, Donaher JL, Bergh J, Liu A, 
            Miu K, Watnick RS, Reinhardt F, McAllister SS, Jacks T, Weinberg RA
  TITLE     Growth-inhibitory and tumor- suppressive functions of p53 depend on 
            its repression of CD44 expression.
  JOURNAL   Cell. 2008 Jul 11. 134(1):62-73.
  PUBMED    18614011
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            Jul 11. 134(1):62-73."
COMMENT     Sequence Label: shCD44-3 pRRL
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    117..234
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        283..793
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     712..730
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      810..819
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggtggaagga cgaaacaccg gttgtaacac ctacaccatt atcctcgagg ataatggtgt
       61 aggtgttaca tttttgaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt
      121 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
      181 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
      241 tattacaggg acagcagaga tccactttgg ccgcggctcg agggggttgg ggttgcgcct
      301 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
      361 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
      421 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
      481 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
      541 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
      601 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
      661 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
      721 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
      781 cctctctccc cagggggatc ccaccggtcg ccaccatggt gagcaagggc gaggagctgt
      841 tcaccggggt ggtgcccatc ctggtc
//
