LOCUS       sequence-116596-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes a shRNA against ERK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Deschenes-Simard X, Gaumont-Leclerc MF, Bourdeau V, Lessard F, 
            Moiseeva O, Forest V, Igelmann S, Mallette FA, Saba-El-Leil MK, 
            Meloche S, Saad F, Mes-Masson AM, Ferbeyre G
  TITLE     Tumor suppressor activity of the ERK/MAPK pathway by promoting 
            selective protein degradation.
  JOURNAL   Genes Dev. 2013 Apr 15;27(8):900-15. doi: 10.1101/gad.203984.112. 
            Epub 2013 Apr 18.
  PUBMED    23599344
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.203984.112"; journalName: "Genes Dev"; date: 
            "2013-04-15- 15"; volume: "27"; issue: "8"; pages: "900-15"
COMMENT     Sequence Label: shERK2b-mlpx-neo
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..833
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(93..112)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     703..722
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 atctccgggc ctttcgacct gcagccaata tgggatcggc cattgaacaa gatggattgc
       61 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
      121 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
      181 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
      241 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
      301 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
      361 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
      421 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
      481 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
      541 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
      601 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
      661 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
      721 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
      781 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tga
//
