LOCUS       sequence-116600-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encoding multiple cloning site, rybozyme and IRES-CAS9..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Yoshioka S, Fujii W, Ogawa T, Sugiura K, Naito K
  TITLE     Development of a mono-promoter-driven CRISPR/Cas9 system in 
            mammalian cells.
  JOURNAL   Sci Rep. 2015 Dec 16;5:18341. doi: 10.1038/srep18341.
  PUBMED    26669567
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-12-16"; volume: "5:18341. doi"; pages: " 
            10.1038/srep18341"
COMMENT     Sequence Label: pMCS-rybozyme-IRES-CAS9
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctcttcttct tggggtcagc cctgctgtct ccaccgagct gagagaggtc gattcttgtt
       61 tcatagagcc ccgtaattga ctgatgaatc agtgtggcgt ccaggacctc ctttgtagag
      121 gtgtaccgct ttctgtctat ggtggtgtcg aagtacttga aggctgcagg cgcgcccaag
      181 ttggtcagag taaacaagtg gataatgttt tctgcctgct ccctgatggg cttatccctg
      241 tgcttattgt aagcagaaag caccttatcg aggttagcgt cggcgaggat cactcttttg
      301 gagaattcgc ttatttgctc gatgatctca tcaaggtagt gtttgtgttg ttccacgaac
      361 agctgcttct gctcattatc ttcgggagac cctttgagct tttcatagtg gctggccaga
      421 tacaagaaat taacgtattt agagggcagt gccagctcgt tacctttctg cagctcgccc
      481 gcactagcga gcattcgttt ccggccgttt tcaagctcaa agagagagta cttgggaagc
      541 ttaatgatga ggtctttttt gacctcttta tatcctttcg cctcgagaaa gtcgatgggg
      601 tttttttcga agcttgatcg ctccatgatt gtgatgccca gcagttcctt gacgcttttg
      661 agttttttag acttcccttt ctccactttg gccacaacca gtacactgta agcgactgta
      721 ggagaatcga atccgccgta tttcttgggg tcccaatctt ttttgcgtgc gatcagcttg
      781 tcgctgttcc ttttcgggag gatactttcc ttggagaagc ctccggtctg tacttcggtc
      841 ttt
//
