LOCUS       sequence-116608-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Outer Mitochondrial Membrane, Excitation: 399, 
            Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-TOMM20-N-10
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(177..887)
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     regulatory      884..893
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
       61 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
      121 gtaaaacctc tacaaatgtg gtatggctga ttatgatcta gagtcgcggc cgcttaatta
      181 agcttgtgcc ccagtttgct agggaggtcg cagtatctgg ccactgccac ctcgtgctgc
      241 tcgacgtagg tctcgttgtt ggcctccttg attctttcca gtctgtagtc cacatagtag
      301 acgccaggca tcttgaggtt cttagcgggt ttcttggatc tatatgtggt cttggcgttt
      361 gcgatcagat ggctcccgcc cacgagcttc agggccatgt cgtttctgcc ttccaggccg
      421 ccgtcagcgg ggtacagcgt ctcggtgaag gcctcccagc cgagtgtttt cttctgcatc
      481 acagggccgt tggatgtgaa gttcacccct ctgatcttga cgttgtagat gaggcagccg
      541 tcctggaggc tggtgtcctg ggtagcggtc agcacgcccc cgtcttcgta tgtggtgact
      601 ctctcccatg tgaagccctc agggaaggac tgcttgaaga agtcggggat gccctgggtg
      661 tggttgatga aggtcttgct gccgtagagg aagctagtag ccaggatgtc gaaggcgaag
      721 gggagagggc cgccctcgac caccttgatt ctcatggtct gggtgccctc gtagggcttg
      781 ccttcgccct cggatgtgca cttgaagtga tggttgtcca cggtgccctc catgtacagc
      841 ttcatgtgca tgttctcctt aatcagctct tcgcccttag acaccatggt ggcgaccggt
      901 ggatccccgc taccgccttc cacatcatct tcagccaagc tctgagcact tacaattctc
      961 tgactaattg t
//
