LOCUS       sequence-116618-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Competitor vector for transcriptional mutagenesis assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   You C, Wang Y
  TITLE     Quantitative measurement of transcriptional inhibition and 
            mutagenesis induced by site-specifically incorporated DNA lesions in
            vitro and in vivo.
  JOURNAL   Nat Protoc. 2015 Sep;10(9):1389-406. doi: 10.1038/nprot.2015.094. 
            Epub 2015 Aug 20.
  PUBMED    26292071
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.094"; journalName: "Nat Protoc"; date: 
            "2015-09"; volume: "10"; issue: "9"; pages: "1389-406"
COMMENT     Sequence Label: pTGFP-T7-Hha10comp
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     142..162
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(187..291)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      318..773
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(405..424)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    780..901
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(791..810)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     845..864
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
       61 gtctcttgat cgatctttgc aaaagcctag gatccatgct ttgcatactt ctgcctgctg
      121 gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc
      181 ctcaggactt ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
      241 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
      301 ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      361 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      421 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
      481 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
      541 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtt
      601 cacgttcttt aatagtggac ttttgttcca aactggaaca acactcaacc ctatctcggt
      661 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
      721 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttacgcctt
      781 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
      841 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
      901 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
//
