LOCUS       sequence-116620-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Hsieh AC, Bo R, Manola J, Vazquez F, Bare O, Khvorova A, Scaringe S,
            Sellers WR
  TITLE     A library of siRNA duplexes targeting the phosphoinositide 3-kinase 
            pathway: determinants of gene silencing for use in cell-based 
            screens.
  JOURNAL   Nucleic Acids Res. 2004  . 32(3):893-901.
  PUBMED    14769947
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2004  . 32(3):893-901."
COMMENT     Sequence Label: 1477 pcDNA3 flag HA Akt1
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             88..111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             124..150
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             124..150
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agcgactctc tggctactga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct taccatggac tacaaggacg acgatgacaa gctcgatgga
      121 ggatacccct acgacgtgcc cgactacgcc agcggatcca tgagcgacgt ggctattgtg
      181 aaggagggtt ggctgcacaa acgaggggag tacatcaaga cctggcggcc acgctacttc
      241 ctcctcaaga atgatggcac cttcattggc tacaaggagc ggccgcagga tgtggaccaa
      301 cgtgaggctc ccctcaacaa cttctctgtg gcgcagtgcc agctgatgaa gacggagcgg
      361 ccccggccca acaccttcat catccgctgc ctgcagtgga ccactgtcat cgaacgcacc
      421 ttccatgtgg agactcctga ggagcgggag gagtggacaa ccgccatcca gactgtggct
      481 gacggcctca agaagcagga ggaggaggag atggacttcc ggtcgggctc acccagtgac
      541 aactcagggg ctgaagagat ggaggtgtcc ctggccaagc ccaagcaccg cgtgaccatg
      601 aacgagtttg agtacctgaa gctgctgggc aagggcactt tcggcaaggt gatcctggtg
      661 aaggagaagg ccacaggccg ctactacgcc atgaagatcc tcaagaagga agtcatcgtg
      721 gccaaggacg aggtggccca cacactcacc gagaaccgcg tcctgcagaa ctccaggcac
      781 cccttcctca cagccctgaa gtactctttc cagacccacg accgcctctg ctttgtcatg
      841 gagtacgcca acgggggcga gctgttcttc cacctgtccc gggaacgtgt
//
