LOCUS       sequence-116636-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes a shRNA against ERK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Deschenes-Simard X, Gaumont-Leclerc MF, Bourdeau V, Lessard F, 
            Moiseeva O, Forest V, Igelmann S, Mallette FA, Saba-El-Leil MK, 
            Meloche S, Saad F, Mes-Masson AM, Ferbeyre G
  TITLE     Tumor suppressor activity of the ERK/MAPK pathway by promoting 
            selective protein degradation.
  JOURNAL   Genes Dev. 2013 Apr 15;27(8):900-15. doi: 10.1101/gad.203984.112. 
            Epub 2013 Apr 18.
  PUBMED    23599344
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.203984.112"; journalName: "Genes Dev"; date: 
            "2013-04-15- 15"; volume: "27"; issue: "8"; pages: "900-15"
COMMENT     Sequence Label: shERK2a-mlpx-puro
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           14..134
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           162..176
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           204..330
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        344..843
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(379..402)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     776..795
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 acagggtaat tgtttgaatg aggcttcagt actttacaga atcgttgcct gcacatcttg
       61 gaaacacttg ctgggattac ttcttcaggt taacccaaca gaaggctcga gaaggtatat
      121 gctgttgaca gtgagcgccc agatcctcag agggttaaat agtgaagcca cagatgtatt
      181 taaccctctg aggatctggt tgcctactgc ctcggaattc aaggggctac tttaggagca
      241 attatcttgt ttactaaaac tgaatacctt gctatctctt tgatacattt ttacaaagct
      301 gaattaaaat ggtataaatt aaatcacttt tttcaattct accgggtagg ggaggcgctt
      361 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
      421 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
      481 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
      541 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
      601 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
      661 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
      721 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
      781 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
      841 tcgacctgca gcc
//
