LOCUS       sequence-116667-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synchronize trafficking of ManII from the ER (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: Ii-Str_ManII-SBP-tagBFP
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(81..194)
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 ttgaagtggt gattatccac ggttccctcc atgtacagtt tcatatgcat gttttccttg
       61 atgagttcag aacctgcagg tggttcacgt tgaccttgtg ggtgatgctc cagtcgtgca
      121 cgaagttgtt caagttcccc agccagtcct tcaacaacgt gtccacctcg ccaaccagtg
      181 gtcttctcgt ccatggaatt ccggtggcga ccggtgggat ccaaacagtc tctggggtca
      241 gcctgcaggg ccaactgtgg cgagtggagg tggatggagc cttggctggc gttgcctggt
      301 gacccccgcg ggccgtcctc cacagactcg ctcaggttga tgactgagtc tctgatattt
      361 gagataatct cgttgttctc agcgagcaaa cgctccaaat ggtcaatctt ttcttgcaat
      421 attgaaagct ggccctgcgg aaaggagccc tcctggcgcg ggccccgagg gtagtccaag
      481 tgacccctgt ccagcatcag gtagagtgag aagattacga cgcagaagat cgcgctgcca
      541 aacacggtga actggcgact taacttcatg gcgcgcctcc cgggttgtgg caagcttatc
      601 atcgtgtttt tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga cgttttttta
      661 acctcgacta aacacatgta aagcatgtgc accgaggccc cagatcagat cccatac
//
