LOCUS       sequence-116669-         580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by 
            mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC43
FEATURES             Location/Qualifiers
     source          1..580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      213..232
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
ORIGIN
        1 gtatcccttg gagaaccacc ttgttggtac gttctctatc actgatagtt taagagctat
       61 gctggaaaca gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc
      121 accgagtcgg tgcgggagct aaggagttta tatggaaacc cttagcctgc tgcgtaagga
      181 gtttatatgg aaacccttac gcagcagttc cctttttttg ttttttatgt ctctgcagag
      241 ttcggtctcg agtactagga tccattaggc ggccgcgtgg ataaccgtat taccgccatg
      301 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      361 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      421 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      481 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      541 gccaagtacg ccccctattg acgtcaatga cggtaaatgg
//
