LOCUS       sequence-116674-         633 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses epsilon COP as a GFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Morohashi Y, Balklava Z, Ball M, Hughes H, Lowe M
  TITLE     Phosphorylation and membrane dissociation of the ARF exchange factor
            GBF1 in mitosis.
  JOURNAL   Biochem J. 2010 Apr 14;427(3):401-12. doi: 10.1042/BJ20091681.
  PUBMED    20175751
REFERENCE   2  (bases 1 to 633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2010-04-14- 14"; volume: "427"; issue: "3"; 
            pages: "401-12"
COMMENT     Sequence Label: pLVXPuro-epsilon-COP-GFP
FEATURES             Location/Qualifiers
     source          1..633
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..630)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(114..134)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     611..630
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gtatccagag gttgattgtt ccagacgcgg tcaggcaccg ggcttgcggg tcatgcacca
       61 ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta
      121 gaaggggagg ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc
      181 cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt ggtcgggcga
      241 gacgccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg ccaggaggcc
      301 ttccatctgt tgctgcgcgg ccagccggga accgctcaac tcggccatgc gcgggccgat
      361 ctcggcgaac accgcccccg cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc
      421 gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc
      481 ttgcagctcg gtgacccgct cgatgtggcg gtccggatcg acggtgtggc gcgtggcggg
      541 gtagtcggcg aacgcggcgg cgagggtgcg tacggccctg gggacgtcgt cgcgggtggc
      601 gaggcgcacc gtgggcttgt actcggtcat ggt
//
