LOCUS       sequence-116676-         594 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Dynein-1 LIC1 as a mGFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 594)
  TITLE     pLVXPuro-mGFP-LIC1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLVXPuro-mGFP-LIC1
FEATURES             Location/Qualifiers
     source          1..594
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      116..125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(122..124,125..127,128..586)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(167..188)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(428..447)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
       61 ccgacatcta ctagaggatc gctagcgcta ccggactcag atctcgacac cggtcgccac
      121 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      181 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      241 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      301 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
      361 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      421 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      481 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      541 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agca
//
