LOCUS       sequence-116681-         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of His-tagged espA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Karpman D, Bekassy ZD, Sjogren AC, Dubois MS, Karmali MA, 
            Mascarenhas M, Jarvis KG, Gansheroff LJ, O'Brien AD, Arbus GS, Kaper
            JB
  TITLE     Antibodies to intimin and Escherichia coli secreted proteins A and B
            in patients with enterohemorrhagic Escherichia coli infections.
  JOURNAL   Pediatr Nephrol. 2002 Mar;17(3):201-11.
  PUBMED    11956862
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Pediatr 
            Nephrol."; date: "2002-03"; volume: "17(3)"; pages: "201-11"
COMMENT     Sequence Label: pCVD468
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(649..666)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             685..696
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggatctatca acaggagtcc aagctcagct aattaagctt ggctgcaggt cgacccgggg
       61 taccggttat ttaccgaggg atattgctga aatagttcta tattgtagag attgcacatc
      121 agaacgtgca ctcgttaaga gatttaatgt attcgacatt tgctgaattt cgagctggct
      181 attattcact accgttgtca ggttattcgc tttagctgaa atagccgcct tcactgtttg
      241 cagatcacca gcgcttaaat caccactaag atcacgaata ccagttacac ttatgtcatt
      301 acgtggatcg tttatatagt caatcacgtc ttgaggaagt ttggctttcg cattcttatc
      361 agtgctactc tgaacatcag caattttggc atccacaaga ttagccatct tttgtgccgt
      421 ggttgacgct ttagatgcct cattcatatc agcaaacttt gcaatcgaca gattactttg
      481 tgcctgatac atataagaaa acatgagaat cgcagcctga aaaacaccga gttcctcaaa
      541 tagcttaacc acctcatcct tcgacatatt acctaagtca tagatcgtcg atgtcgaaga
      601 actcgcactc acattaacaa cggatgttgc atttgatgta tcggatccgt gatggtgatg
      661 gtgatgcgat cctctcatag ttaatttctc ctctttaatg aattctgtgt gaa
//
