LOCUS       sequence-116682-         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for express truncated form of Sec16A as a fusion 
            with Venus FP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Hughes H, Stephens DJ
  TITLE     Sec16A defines the site for vesicle budding from the endoplasmic 
            reticulum on exit from mitosis.
  JOURNAL   J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8. Epub 2010 Nov 2.
  PUBMED    21045114
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2010-12-1"; volume: "123(Pt 23)"; pages: "4032-8. Epub
            2010 Nov 2"
COMMENT     Sequence Label: pSEW-Venus-Sec16A-deltaN
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(414..434)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          564..629
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
       61 aaacctctac aaatgtggta tggctgatta tgatctctag tcaaggcact atacatcaaa
      121 tattccttat taaccccttt acaaattaaa aagctaaagg tacacaattt ttgagcatag
      181 ttattaatag cagacactct atgcctgtgt ggagtaagaa aaaacagtat gttatgatta
      241 taactgttat gcctacttat aaaggttaca gaatattttt ccataatttt cttgtatagc
      301 agtgcagctt tttcctttgt ggtgtaaata gcaaagcaag caagagttct attactaaac
      361 acagcatgac tcaaaaaact tagcaattct gaaggaaagt ccttggggtc ttctaccttt
      421 ctcttctttt ttggaggagt agaatgttga gagtcagcag tagcctcatc atcactagat
      481 ggcatttctt ctgagcaaaa caggttttcc tcattaaagg cattccacca ctgctcccat
      541 tcatcagttc cataggttgg aatctaaaat acacaaacaa ttagaatcag tagtttaaca
      601 cattatacac ttaaaaattt tatatttacc ttagagcttt aaatctctgt aggtag
//
