LOCUS       sequence-116690-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of near-infrared PI(4,5)P2 biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Idevall-Hagren O, Dickson EJ, Hille B, Toomre DK, De Camilli P
  TITLE     Optogenetic control of phosphoinositide metabolism.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):E2316-23. doi: 
            10.1073/pnas.1211305109. Epub 2012 Jul 30.
  PUBMED    22847441
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1211305109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-08-28- 28"; volume: "109"; issue: "35"; pages: 
            "E2316-23"
COMMENT     Sequence Label: iRFP-PH-PLCdelta1
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(46..249)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(75..95)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        250..553
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(808..827)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggatccttcg ccatggtgct agcggatctg acggttcact aaaccagctc tgcttatata
       61 gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca
      121 ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg
      181 gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa
      241 ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc
      301 ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat
      361 agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa
      421 atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt
      481 cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt
      541 aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta
      601 ctattaataa ctaatgcatg gcggtaatac ggttatccac agaatcaggg gataacgcag
      661 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      721 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      781 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      841 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      901 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      961 ttcgctccaa gctgggctgt gtgcacgaac ccc
//
