LOCUS       sequence-116703-         584 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses human MyoD and dsRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Gersbach C
  TITLE     Multiplex CRISPR/Cas9 Genome Engineering for Directing Myogenic 
            Cellular Differentiation in Primary Human Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV hMyoD-IRES-dsRed
FEATURES             Location/Qualifiers
     source          1..584
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(105..128)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(303..322)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     520..540
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 gtaatccaga ggttgattat cgataagctt gatatcgaat tcctactgga acaggtggtg
       61 gcgggcctcg gcgcgctcgt actgctccac cacggtgtag tcctcgttgt gggaggtgat
      121 gtccagcttg gagtccacgt agtagtagcc gggcagcttc acgggcttct tggccatgta
      181 gattgacttg aactccacca ggtagtggcc gccgcccttc agcttcagcg ccttgtggat
      241 ctcgcccttc agcacgccgt cgcgggggta caggcgctcg gtggagggct cccagcccag
      301 agtcttcttc tgcattacgg ggccgtcgga ggggaagttc acgccgatga acttcacgtg
      361 gtagatgaag gtgccgtcct gcagggagga gtcctgggtc acggtcacca cgccgccgtc
      421 ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct tcttgtagtc
      481 ggggatgtcg gcggggtgct tcacgtacac cttggagccg tactggaact ggggggacag
      541 gatgtcccag gcgaagggca gggggccgcc cttggtcacc tgca
//
