LOCUS       sequence-116709-         458 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expresses CRE excisable human MyoD and dsRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Gersbach C
  TITLE     Multiplex CRISPR/Cas9 Genome Engineering for Directing Myogenic 
            Cellular Differentiation in Primary Human Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV floxed hMyoD-IRES-dsRed
FEATURES             Location/Qualifiers
     source          1..458
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..71
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(140..163)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(338..357)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtaatccaga ggttgattat cgataagctt gatatccata acttcgtata atgtatgcta
       61 tacgaagtta tgaattccta ctggaacagg tggtggcggg cctcggcgcg ctcgtactgc
      121 tccaccacgg tgtagtcctc gttgtgggag gtgatgtcca gcttggagtc cacgtagtag
      181 tagccgggca gcttcacggg cttcttggcc atgtagattg acttgaactc caccaggtag
      241 tggccgccgc ccttcagctt cagcgccttg tggatctcgc ccttcagcac gccgtcgcgg
      301 gggtacaggc gctcggtgga gggctcccag cccagagtct tcttctgcat tacggggccg
      361 tcggagggga agttcacgcc gatgaacttc acgtggtaga tgaaggtgcc gtcctgcagg
      421 gaggagtcct gggtcacggt caccacgccg ccgtcctc
//
