LOCUS       sequence-116710-         647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expresses CRE excisable human MyoD and dsRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Gersbach C
  TITLE     Multiplex CRISPR/Cas9 Genome Engineering for Directing Myogenic 
            Cellular Differentiation in Primary Human Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV floxed hMyoD-IRES-dsRed
FEATURES             Location/Qualifiers
     source          1..647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(62..95)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        125..524
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
       61 tataacttcg tatagcatac attatacgaa gttatgttaa ttaacccgtg tcggctccag
      121 atctggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc
      181 gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg
      241 acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt
      301 ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc
      361 gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc
      421 cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt
      481 gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg
      541 ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga
      601 ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc ctgaact
//
