LOCUS       sequence-116747-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: , Emission: .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Davidson Lab
  TITLE     FRET Indicators - Live and Fixed Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mTurquoise-Clover-FRET-10
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(193..444)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(235..256)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     584..603
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gctttatttg tgaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
       61 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
      121 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atctagagtc
      181 gcggccgctt tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
      241 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcagct tggactgggt
      301 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
      361 gtagtggtcg gcgagctgca cgccgccgtc ctcgatgttg tggcggatct tgaagttggc
      421 cttgatgccg ttcttctgct tgtcggcggt gatatagacg ttgtcgctga tgtagttgta
      481 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
      541 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
      601 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
      661 gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac tgcacgcccc aggacagggt
      721 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
      781 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
      841 tacgt
//
