LOCUS       sequence-116788-         676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SUMO2(Q87R) with a strep and an HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Bursomanno S, McGouran JF, Kessler BM, Hickson ID, Liu Y
  TITLE     Regulation of SUMO2 target proteins by the proteasome in human cells
            exposed to replication stress.
  JOURNAL   J Proteome Res. 2015 Apr 3;14(4):1687-99. doi: 10.1021/pr500997p. 
            Epub 2015 Mar 19.
  PUBMED    25748227
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr500997p";
            journalName: "J Proteome Res"; date: "2015-04-3- 3"; volume: "14"; 
            issue: "4"; pages: "1687-99"
COMMENT     Sequence Label: pcDNA3.1-StrepHA-SUMO2(Q87R)
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             93..116
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             153..176
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             204..230
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             246..524
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 2"
                     /label=SUMO2
                     /translation="MADEKPKEGVKTENNNHINLKVAGQDGSVVQFKIKRHTPLSKLMK
                     AYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFRQQTGG"
     primer_bind     complement(578..595)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
       61 cgtttaaact taagcttggt accatggcta gctggagcca cccgcagttt gagaaaggtg
      121 gaggttccgg aggtggatcg ggaggtggat cgtggagcca cccgcagttc gaaaaaggcg
      181 ccgagaccgc ggtcccgaat tcttaccctt atgacgtccc cgattacgcc gctgcagcgg
      241 atatcatggc cgacgaaaag cccaaggaag gagtcaagac tgagaacaac aatcatatta
      301 atttgaaggt ggcggggcag gatggttctg tggtgcagtt taagattaag aggcatacac
      361 cacttagtaa actaatgaaa gcctattgtg aacgacaggg attgtcaatg aggcagatca
      421 gattccgatt tgacgggcaa ccaatcaatg aaacagacac acctgcacag ttggaaatgg
      481 aggatgaaga tacaattgat gtgttccgac agcagacggg aggtgtctac tgagcggccg
      541 ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
      601 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
      661 actgtccttt cctaat
//
