LOCUS       sequence-116791-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a light-inducible PI3-kinase, generated by fusing the 
            iSH2 domain of human p85-alpha (residues 420–615) to mCherry-CRY2. 
            Binds endogenous PI3-kinase p110-alpha catalytic subunits 
            constitutively..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Idevall-Hagren O, Dickson EJ, Hille B, Toomre DK, De Camilli P
  TITLE     Optogenetic control of phosphoinositide metabolism.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):E2316-23. doi: 
            10.1073/pnas.1211305109. Epub 2012 Jul 30.
  PUBMED    22847441
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1211305109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-08-28- 28"; volume: "109"; issue: "35"; pages: 
            "E2316-23"
COMMENT     Sequence Label: mCherry-CRY2-iSH2
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..29
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
       61 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
      121 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcagttatct
      181 agatccggtg gatcccgggc ccgcggtacc tcaatcctca tcatcttcta ccagtgagta
      241 ttggtcttct gtgttttcgt ttcccaacca ttcgttcagc ttcttctgcc ggaccccttt
      301 ctgcgtcagc cacatcaagt attgatctct tgtctttctc agctgaatga ggtccggctt
      361 aatgctattc atgcgcttgt cgatctcgcg atactcggct gcctgcttct tcaagtcttc
      421 ctccagtctc ctcctgctgt cgatgatctc gctgatccga gacttcagct tatcgtgatt
      481 atgcattatc ctttgaattt ccttctcgtt gccttcgcgt ttaaacttct ctatgtattc
      541 tttgctgtac cgctcctggg tttggcactg ctcttcgaat atttttatgg tttcattaaa
      601 tgcttcgata gctgttcttt tcatctgtat ttcctgggaa gtacgagtgt attcctcata
      661 taatctatca tattcccgac ttttttcttg aaattgagta ttatattcat gtaatttttt
      721 ccctacagct tcaatattat cttctttgac aacttgatcc tgctggtatt tagacactgg
      781 gtagagcaac ttcacatcca gcgatcgacc tgctcctcct gctcccgctg ctgcgcctcg
      841 agctgctgct ccgatcatga tctgtgcttc acgggttctt gaaatagctt tagctagtag
      901 ctcacgagct gtgtcgatgt ctacaatggg tttcgcatag tttgttccga gttccacacc
      961 agaagctttg agtacggtta aag
//
