LOCUS       sequence-116803-         633 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CFP fusion of Sec23A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Stephens DJ, Lin-Marq N, Pagano A, Pepperkok R, Paccaud JP
  TITLE     COPI-coated ER-to-Golgi transport complexes segregate from COPII in 
            close proximity to ER exit sites.
  JOURNAL   J Cell Sci. 2000 Jun;113 ( Pt 12):2177-85.
  PUBMED    10825291
REFERENCE   2  (bases 1 to 633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2000-06"; volume: "113 ( Pt 12)"; pages: "2177-85"
COMMENT     Sequence Label: pECFP-Sec23A
FEATURES             Location/Qualifiers
     source          1..633
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(40..42,43..45,46..558)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(85..106)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(346..365)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gtcgccacca tggtgagcaa gggcgaggag
       61 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacagg
      121 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      181 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg
      241 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      301 gccatgcccg aaggctacgt ccaggagcgt accatcttct tcaaggacga cggcaactac
      361 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      421 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc
      481 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc ccacttcaag
      541 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
      601 cccatcggcg acggccccgt gctgctgccc gac
//
