LOCUS       sequence-116815-         515 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Theophylline Riboswitch B with lacZ gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Topp S, Reynoso CM, Seeliger JC, Goldlust IS, Desai SK, Murat D, 
            Shen A, Puri AW, Komeili A, Bertozzi CR, Scott JR, Gallivan JP
  TITLE     Synthetic riboswitches that induce gene expression in diverse 
            bacterial species.
  JOURNAL   Appl Environ Microbiol. 2010 Dec;76(23):7881-4. doi: 
            10.1128/AEM.01537-10. Epub 2010 Oct 8.
  PUBMED    20935124
REFERENCE   2  (bases 1 to 515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.01537-10"; journalName: "Appl Environ Microbiol"; date:
            "2010-12"; volume: "76"; issue: "23"; pages: "7881-4"
COMMENT     Sequence Label: pBAV-B
FEATURES             Location/Qualifiers
     source          1..515
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(281..295,296..301,302..318,319..325)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    301..317
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    333..349
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctatcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
       61 cctcttttct acggtattta aagatacccc aagaagctaa ttataacaag acgaactcca
      121 agttcactgt tccttgcgtt ctagcaacct tacatacaga aaacagcctt ttcaaagttg
      181 ttttcaaagt tggcgtatgc catagtatcg acggagccga ttttgaaccc acacttatga
      241 tagaatttga cgtcgaattc gcggccgctt ctcgaggaaa tcataaaaaa tttatttgct
      301 ttgtgagcgg ataacaatta taatagattc aattgtgagc ggataacaat tactagagat
      361 acgactcact ataggtaccg gtgataccag catcgtcttg atgcccttgg cagcacccgc
      421 tgcgcagggg gtatcaacaa gatgtgcgaa ctcgatattt tacgcgactc tctttaccaa
      481 ttctgccccg aattacactt gaaacgactc aactg
//
