LOCUS       sequence-116822-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-ER-5
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             94..747
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 tgaaccgtca gatccgctag cgccaccatg ctgctatccg tgccgttgct gctcggcctc
       61 ctcggcctgg ccgtcgccgc accggtcgcc accatggtga gtgtgattaa accagagatg
      121 aagatgaggt actacatgga cggctccgtc aatgggcatg agttcacaat tgaaggtgaa
      181 ggcacaggca gaccttacga gggacatcaa gagatgacac tacgcgtcac aatggccgag
      241 ggcgggccaa tgcctttcgc gtttgactta gtgtcacacg tgttctgtta cggccacaga
      301 gtatttacta aatatccaga agagatacca gactatttca aacaagcatt tcctgaaggc
      361 ctgtcatggg aaaggtcgtt ggagttcgaa gatggtgggt ccgcttcagt cagtgcgcat
      421 ataagcctta gaggaaacac cttctaccac aaatccaaat ttactggggt taactttcct
      481 gccgatggtc ctatcatgca aaaccaaagt gttgattggg agccatcaac cgagaaaatt
      541 actgccagcg acggagttct gaagggtgat gttacgatgt acctaaaact tgaaggaggc
      601 ggcaatcaca aatgccaaat gaagactact tacaaggcgg caaaagagat tcttgaaatg
      661 ccaggagacc attacatcgg ccatcgcctc gtcaggaaaa ccgaaggcaa cattactgag
      721 caggtagaag atgcagtagc tcatagctcc ggaaaggacg agctgtaaga attctgcagt
      781 cgacggtacc gcgggcccgg gatccaccgg atctagataa ctg
//
