LOCUS       sequence-116834-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mOct4 reporter plasmid (expresses CNL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pRZ-mOct4-CNL
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(365..367,368..370,371..883)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(410..431)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(671..690)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgat ggcagatgca
       61 taacaaaggc cctgcccctc cccccaggga ggttgagagt tctgggcaga cggcagatgc
      121 ataacaaagg tgcatgccct gcccctcccc ccagggaggt tgagagttct gggcagacgg
      181 cagatgcata acaaaggtgc atggggtgtg gggaggttgt agcccgaccc tgcccctccc
      241 cccagggagg ttgagagttc tgggcagacg gcagatgcat aacaaaggtg catgatagct
      301 ctgccctggg ggcagagaag atggttgggg aggggtccct ctcgtcctag tcggatccac
      361 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      421 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      481 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      541 caccctcgtg accaccctgt cctggggcgt gcagtgcttc gcccgctacc ccgaccacat
      601 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      661 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      721 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      781 gcacaagctg gagtacaact actttagcga caacgtctat atcaccgccg acaagcagaa
      841 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct
      901 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
      961 ccactacctg agcacccagt ccaagctgag caa
//
