LOCUS       sequence-116837-        1131 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mSox2 reporter plasmid (expresses ONL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pGF-mSox2-ONL
FEATURES             Location/Qualifiers
     source          1..1131
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             357..1010
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             357..1010
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgat gaattctaat
       61 taatgcagag actctaaaag aatttcccgg gctcgggcag ccattgtgat gcatatagga
      121 ttattcacgt ggtaatgtaa ttaatgcaga gactctaaaa gaatttcccg ggctcgggca
      181 gccattgtga tgcatatagg attattcacg tggtaatgcc agtccaagct aggcaggttc
      241 ccctctaatt aatgcagaga ctctaaaaga atttcccggg ctcgggcagc cattgtgatg
      301 catataggat tattcacgtg gtaatgagca cagtcgacag ttcttgcgga tccaccatgg
      361 tgagcgtgat caagcccgag atgaagatga ggtactacat ggacggctcc gtcaatgggc
      421 atgagttcac aatcgagggt gagggcacag gcagacctta cgagggacat caggagatga
      481 cactgcgcgt cacaatggcc gagggcgggc caatgccttt cgccttcgac ctggtgtccc
      541 acgtgttctg ttacggccac agagttttta ctaaatatcc agaagagatc ccagactatt
      601 tcaagcaggc ctttcctgag ggcctgtcct gggagaggtc cctggagttc gaggacggcg
      661 gctccgcctc cgtgagcgcc cacatcagcc tgaggggcaa caccttctac cacaagtcca
      721 agttcaccgg cgtgaacttc cccgccgacg gccccatcat gcagaaccag agcgtggact
      781 gggagccctc caccgagaag atcaccgcca gcgacggcgt gctgaagggc gacgtgacca
      841 tgtacctgaa gctggagggc ggcggcaacc acaagtgcca gatgaagacc acctacaagg
      901 ccgccaagga gatcctggag atgcccggcg accactacat cggccacagg ctggtgagga
      961 agaccgaggg caacatcacc gagcaggtgg aggacgccgt ggcccactcc ggtaccaagg
     1021 tgtacgaccc cgagcaacgc aaacgcatga tcactgggcc tcagtggtgg gctcgctgca
     1081 agcaaatgaa cgtgctggac tccttcatca actactatga ttccgagaag c
//
