LOCUS       sequence-116848-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CNL-fibrillarin in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pCNL-C1_fibrillarin
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(51..53,54..56,57..569)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(96..117)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(357..376)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     704..725
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgccacc atggtgagca
       61 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      121 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      181 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      241 ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
      301 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      361 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      421 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      481 acaactactt tagcgacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
      541 ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc
      601 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
      661 cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
      721 tcgtgaccgc cgccgggggt accaaggtgt acgaccccga gcagaggaag aggatgatca
      781 ccggccccca gtggtgggcc aggtgcaagc agatgaacgt gctggacagc ttcatcaact
      841 actacgacag cgagaagcac gccgagaacg ccgtgatctt cctgcacggc aacgccacta
      901 gcagctacct gtggaggcac gtggtgcccc acatcgagcc cgtggccagg tgcatcatcc
      961 ccgatctgat cggcatgggc aagagcggca agagcggcaa cggcagctac aggctgctgg
     1021 accactacaa gtacctgacc gcctggttcg agcttctgaa cctgcccaag aagatcatct
     1081 tcgtgg
//
