LOCUS       sequence-116850-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ONL-fibrillarin in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pONL-C1_fibrillarin
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             51..704
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             51..704
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgccacc atggtgagcg
       61 tgatcaagcc cgagatgaag atgaggtact acatggacgg ctccgtcaat gggcatgagt
      121 tcacaatcga gggtgagggc acaggcagac cttacgaggg acatcaggag atgacactgc
      181 gcgtcacaat ggccgagggc gggccaatgc ctttcgcctt cgacctggtg tcccacgtgt
      241 tctgttacgg ccacagagtt tttactaaat atccagaaga gatcccagac tatttcaagc
      301 aggcctttcc tgagggcctg tcctgggaga ggtccctgga gttcgaggac ggcggctccg
      361 cctccgtgag cgcccacatc agcctgaggg gcaacacctt ctaccacaag tccaagttca
      421 ccggcgtgaa cttccccgcc gacggcccca tcatgcagaa ccagagcgtg gactgggagc
      481 cctccaccga gaagatcacc gccagcgacg gcgtgctgaa gggcgacgtg accatgtacc
      541 tgaagctgga gggcggcggc aaccacaagt gccagatgaa gaccacctac aaggccgcca
      601 aggagatcct ggagatgccc ggcgaccact acatcggcca caggctggtg aggaagaccg
      661 agggcaacat caccgagcag gtggaggacg ccgtggccca ctccggtacc aaggtgtacg
      721 accccgagca acgcaaacgc atgatcactg ggcctcagtg gtgggctcgc tgcaagcaaa
      781 tgaacgtgct ggactccttc atcaactact atgattccga gaagcacgcc gagaacgccg
      841 tgatttttct gcatggtaac gctacctcca gctacctgtg gaggcacgtc gtgcctcaca
      901 tcgagcccgt ggctagatgc atcatccctg atctgatcgg aatgggtaag tccggcaaga
      961 gcgggaatgg ctcatatcgc ctcctggatc actacaagta cctcaccgct tggttcgagc
     1021 tgctgaacct tccaa
//
